Journal: PLoS ONE

Article Title: Identification of Novel Biomarkers in Seasonal Allergic Rhinitis by Combining Proteomic, Multivariate and Pathway Analysis

doi: 10.1371/journal.pone.0023563

Figure Lengend Snippet: Nasal fluid proteins enriched in the acute phase response pathway .

Article Snippet: Histidine-rich glycoprotein (HRG) was analyzed with an ELISA kit from Cusabio Biotech Co., Ltd (Wuhan, China).

Techniques: Clinical Proteomics

Journal: Clinical Proteomics

Article Title: Decreased histidine-rich glycoprotein and increased complement C4-B protein levels in follicular fluid predict the IVF outcomes of recurrent spontaneous abortion

doi: 10.1186/s12014-022-09383-9

Figure Lengend Snippet: Representive MS/MS spectra of peptides of Clusterin (CLU) ( A ), Histidine-rich glycoprotein (HRG) ( B ), Alpha-1-antitrypsin (SERPINA1) ( C ), Complement C4-B (C4B) ( D ), Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A) ( E ) and Low affinity immunoglobulin gamma Fc region receptor II-a (FCGR2A) ( F ). The right column represented the ions labelled by TMT reagent

Article Snippet: The ELISA kit included histidine-rich glycoprotein (HRG) (SEC534Hu, Cloud-Clone Corp. , USA) and human complement C4-B (C4B) (SEB305Hu, Cloud-Clone Corp., USA).

Techniques: Tandem Mass Spectroscopy

Journal: Clinical Proteomics

Article Title: Decreased histidine-rich glycoprotein and increased complement C4-B protein levels in follicular fluid predict the IVF outcomes of recurrent spontaneous abortion

doi: 10.1186/s12014-022-09383-9

Figure Lengend Snippet: Validation of HRG and C4B expression in enlarged RSA and Control gruop patients. A ELISA validation of HRG expression in 41 RSA and 41 Control group patients. B ROC analysis of HRG. C ELISA validation of C4B expression in 36 RSA and 36 Control group patients. C , D Receiver operating characteristic (ROC) curves of HRG and C4B respectively. The test variable was protein expression level and the classification variable was the outcome of the current IVF cycle. E The ROC curve of HRG and C4B combined, the test variable was the predicted probability calculated by logistic regression of C4B and HRG in the same FF sample. The blue solid line represents the ROC curve. The diagonal line (red dashed line) from [0, 0] to [1, 1] is the line of equivalence. The p -value and area under the curve (AUC) was calculated and shown in the lower right corner of each graph

Article Snippet: The ELISA kit included histidine-rich glycoprotein (HRG) (SEC534Hu, Cloud-Clone Corp. , USA) and human complement C4-B (C4B) (SEB305Hu, Cloud-Clone Corp., USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Clinical Proteomics

Article Title: Decreased histidine-rich glycoprotein and increased complement C4-B protein levels in follicular fluid predict the IVF outcomes of recurrent spontaneous abortion

doi: 10.1186/s12014-022-09383-9

Figure Lengend Snippet: Correlation between HRG and C4B protein expression level and clinical parameters

Article Snippet: The ELISA kit included histidine-rich glycoprotein (HRG) (SEC534Hu, Cloud-Clone Corp. , USA) and human complement C4-B (C4B) (SEB305Hu, Cloud-Clone Corp., USA).

Techniques: Expressing